The Hypoglycemia Effect of Alkaloid Compounds from Oil Free Mahagony Seeds (Swietenia macrophylla, King)

Aim: To study the hypoglycemia effect of alkaloid compounds (Alkaloid crude extract) from oil free mahagony seeds (Swietenia macrophylla, King).

Study Design: Experiments of alkaloid compounds isolation and in vivo test for hypoglycemia effect.

Place and Duration of Study: Sample Mahagony seeds of species S. macrophylla, King obtained from Central Java concession plants between May 2013 and July 2013.

Methodology: The oil of mahagony seeds was isolated by maceration using n-hexane for 3 x 24 hours. Alkaloid compounds from oil free mahagony seeds was carried out using methanol and 10% acetic acid solutions. Test of hypoglycemia effect treated on wistar rats. Before treatment with alkaloid compounds, the rats were given no food for 24 hours. Negative control was aquadest treatment and positive control was glibenclamide treatment. The dose of testing material were 5 mg/kg.rat-wght., 10 mg/kg.rat-wght., and 20 mg/kg.rat-wght. Data collection was performed by measuring UV absorption at λ 630 nm of the blood serum at 30, 60, 90, 120, 150 minutes after the treatment.

Results: The test of hypoglycemia effect was t-test in 5% significancy level, and t- table value at 2.021, showed that significant differences of blood glucose level before and after treatment with alkaloid compounds of 5 mg/kg.rat-wght and 10 mg/kg.rat-wght.

Conclusion: Alkaloid compounds had the hypoglycemia effect.