Molecular Detection of Some Virulence Genes and Determination the Inhibitory Efficiency of Nano Hybrid Antibiotic against (Escherichia coli) Isolated from Urinary Tract Infection

Objective: Escherichia coli is considered the most common microorganism in pathological samples related to urinary tract infections especially in women and children. It contains many virulence factors helping in pathogenicity However it is sensitive to fosfomycin that has broad spectrum in its activity against bacteria which its activity also could be confirmed using nanobiotechnology.

Methods: The study included 25 isolates of E. coli from patients with urinary tract infections. DNA extracted and PCR technique used for detection of 3 genes (afa, pap and fimh) coding for virulence factor afimbrial adhesions proteins, pilus rod and Type 1 fimbriae respectively. Magnesium oxide was used as a carrier in intercalating of fosfomycin between its layers. fourier transform infrared spectroscopy (FT-IR) atomic force microscope (AFM) and X-ray diffraction (XRD) were used for characterizing of prepared nanohybrid antibiotic. Antibacterial activity of against E. coli has been tested.

Results: PCR results showed bands with 750, 328 and 508 bps for the three genes afa, pap and fimh respectively. The all of three techniques used to confirm the hybrating proccess indicated formation of new compound through the appearance of new group of spectrums in FT-IR, formation of spherical nanoparticles in AFM and appearance of a new diffraction planes in XRD.

Conclusion: It can be concluded that E. coli isolate under study were virulent due the presence of virulence genes. Using nanotechnology, Fosfomycin can be used to prepare nanohybrid antibiotic by loading on Magnesium oxide. The prepared nano-particle was efficient in inhibiting E. coli isolated from urinary tract infections.